Cryo-EM dataset of P.berghei kinesin-8B motor domain in AMPPNP state bound to tubulin dimer
P. berghei kinesin-8B motor domain in AMPPNP state bound to tubulin dimer
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7z2b
Q-score: 0.367
Liu T, Shilliday F, Cook AD, Zeeshan M, Brady D, Tewari R, Sutherland CJ, Roberts AJ, Moores CA
Nat Commun (2022) 13 pp. 6988-6988 [ DOI: doi:10.1038/s41467-022-34710-x Pubmed: 36384964 ]
- Tubulin beta chain (47 kDa, Protein from pig)
- Detyrosinated tubulin alpha-1b chain (47 kDa, Protein from pig)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Kinesin-8, putative (38 kDa, Protein from Plasmodium berghei)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- P. berghei kinesin-8b motor domain in amppnp state bound to tubulin dimer (Complex from Plasmodium berghei)
- Magnesium ion (24 Da, Ligand)
Liu T, Shilliday F, Cook AD, Tewari R, Sutherland CJ, Roberts AJ, Moores CA
bioRxiv (2022) [ DOI: 10.1101/2022.02.11.480087 ]
Image sets:
Cryo-EM dataset of P.berghei kinesin-8B motor domains in no nucleotide state bound to tubulin dimer
Liu T, Shilliday F, Cook AD, Tewari R, Sutherland CJ, Roberts AJ, Moores CA
bioRxiv (2022) [ DOI: 10.1101/2022.02.11.480087 ]
Image sets:
P. berghei kinesin-8B motor domain in no nucleotide state bound to tubulin dimer
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7z2a
Q-score: 0.253
Liu T, Shilliday F, Cook AD, Zeeshan M, Brady D, Tewari R, Sutherland CJ, Roberts AJ, Moores CA
Nat Commun (2022) 13 pp. 6988-6988 [ DOI: doi:10.1038/s41467-022-34710-x Pubmed: 36384964 ]
- Tubulin beta chain (47 kDa, Protein from pig)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- P. berghei kinesin-8b motor domain in no nucleotide state bound to tubulin dimer (Complex from Plasmodium berghei)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Kinesin-8, putative (39 kDa, Protein from Plasmodium berghei)
- Detyrosinated tubulin alpha-1b chain (47 kDa, Protein from Pig)
- Magnesium ion (24 Da, Ligand)
P. falciparum kinesin-8B motor domain in no nucleotide bound to tubulin dimer
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7z2c
Q-score: 0.296
Liu T, Shilliday F, Cook AD, Zeeshan M, Brady D, Tewari R, Sutherland CJ, Roberts AJ, Moores CA
Nat Commun (2022) 13 pp. 6988-6988 [ DOI: doi:10.1038/s41467-022-34710-x Pubmed: 36384964 ]
- Tubulin beta chain (47 kDa, Protein from pig)
- Detyrosinated tubulin alpha-1b chain (47 kDa, Protein from pig)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Kinesin-like protein (39 kDa, Protein from Plasmodium falciparum)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- P. falciparum kinesin-8b motor domain in no nucleotide state bound to tubulin dimer (Complex from Plasmodium berghei)
Resolution: 4.4 Å
EM Method: Helical reconstruction
Fitted PDBs: 7nb8
Q-score: 0.268
Cook AD, Roberts AJ, Atherton J, Tewari R, Topf M, Moores CA
J Biol Chem (2021) 297 pp. 101063-101063 [ Pubmed: 34375637 DOI: doi:10.1016/j.jbc.2021.101063 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Alpha/beta-tubulin (Complex from Sus scrofa)
- Kinesin-5 (Complex from Plasmodium falciparum (isolate 3D7))
- 14 protofilament microtubule of alpha/beta-tubulin dimers, with kinesin-5 motor domain bound 1:1 to tubulin dimers. (Complex)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Kinesin-5 (46 kDa, Protein from Plasmodium falciparum (isolate 3D7))
- Tubulin beta chain (49 kDa, Protein from Pig)
- Tubulin alpha-1b chain (50 kDa, Protein from Pig)
- Magnesium ion (24 Da, Ligand)
Resolution: 4.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 7nba
Q-score: 0.268
Cook AD, Roberts AJ, Atherton J, Tewari R, Topf M, Moores CA
J Biol Chem (2021) 297 pp. 101063-101063 [ Pubmed: 34375637 DOI: doi:10.1016/j.jbc.2021.101063 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Kinesin motor domain-containing protein,kinesin motor domain-containing protein (46 kDa, Protein from Plasmodium falciparum (isolate NF54))
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Pig)
- Tubulin alpha-1b chain (50 kDa, Protein from Pig)
- 14 protofilament microtubule complexed with plasmodium falciparum kinesin-5 motor domain bound to amppnp. asymmetric unit. (Complex from Plasmodium falciparum (isolate NF54))
Human kinesin-5 motor domain in the GSK-1 state bound to microtubules
Resolution: 3.8 Å
EM Method: Helical reconstruction
Fitted PDBs: 6ta3
Q-score: 0.215
Pena A, Sweeney A, Cook AD, Topf M, Moores CA
Structure (2020) 28 pp. 450-457.e5 [ Pubmed: 32084356 DOI: doi:10.1016/j.str.2020.01.013 ]
- Kinesin-like protein kif11 (43 kDa, Protein from Homo sapiens)
- Ternary complex of human kinesin 5 and microtubules (Complex)
- 6-[4-(trifluoromethyl)phenyl]-3,4-dihydro-1~{h}-quinolin-2-one (291 Da, Ligand)
- Tubulin beta chain (48 kDa, Protein from Pig)
- Tubulin alpha-1b chain (48 kDa, Protein from Pig)
- Magnesium ion (24 Da, Ligand)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Kinesin-like protein kif11 (Complex from Homo sapiens)
- Tubulin alpha-1b chain and tubulin beta chain (Complex from Sus scrofa)
Human kinesin-5 motor domain in the AMPPNP state bound to microtubules
Resolution: 6.1 Å
EM Method: Helical reconstruction
Fitted PDBs: 6ta4
Q-score: 0.156
Pena A, Sweeney A, Cook AD, Topf M, Moores CA
Structure (2020) 28 pp. 450-457.e5 [ Pubmed: 32084356 DOI: doi:10.1016/j.str.2020.01.013 ]
- Kinesin-like protein kif11 (43 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Amppnp bound human eg5 motor domain in complex with alpha/beta tubulin (Complex from Sus scrofa)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Amppnp bound human eg5 motor domain in complex with alpha/beta tubulin (Complex from Homo sapiens)
- Tubulin beta chain (48 kDa, Protein from Pig)
- Tubulin alpha-1b chain (48 kDa, Protein from Pig)
- Magnesium ion (24 Da, Ligand)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Amppnp bound human eg5 motor domain in complex with alpha/beta tubulin (Complex)